Successful vitrification of bovine and human ovarian tissue
نویسندگان
چکیده
منابع مشابه
Successful vitrification of bovine and human ovarian tissue.
Ovariectomy and ovarian tissue cryopreservation has the potential to preserve the natural fertility of cancer patients prior to sterilizing chemo- and radiotherapies. Ovarian tissue cryopreservation with the conventional slow-freezing method has yielded limited success, partly because of oocyte loss during freeze-thaw and subsequent transplant. Based on the high-efficiency vitrification Cryotop...
متن کاملSuccessful vitrification and autografting of baboon (Papio anubis) ovarian tissue.
STUDY QUESTION Can a vitrification protocol using an ethylene glycol/dimethyl sulphoxide-based solution and a cryopin successfully cryopreserve baboon ovarian tissue? SUMMARY ANSWER Our results show that baboon ovarian tissue can be successfully cryopreserved with our vitrification protocol. WHAT IS KNOWN ALREADY Non-human primates have already been used as an animal model to test vitrifica...
متن کاملChapter 13 Human Ovarian Tissue Vitrification.
Ovarian freezing and transplantation has garnered increasing interest as a potential way of preserving fertility in cancer patients as well as for women who just wish to delay childbearing. This chapter spells out our techniques of ovarian cortex vitrification and results for frozen compared to fresh ovarian cortex transplantation (in one single series from one center for the sake of consistenc...
متن کاملHuman ovarian tissue: vitrification versus conventional freezing.
Sir, In the challenging paper by Wang et al. (2008), the authors have reported about an effective method of vitrification of human ovarian tissue with direct contact of cells in liquid nitrogen, named needle immersed vitrification. Childbirth after cryopreservation of ovarian tissue is now a reality (Donnez et al., 2004; Demeestere et al., 2007; Meirow et al., 2007; Andersen et al., 2008) and t...
متن کاملI-10: Vitrification of Baboon Ovarian Tissue:Will Vitrification Replace Conventional Freezing for Ovarian Tissue Cryopreservation?
Background: The aim of this study was to evaluate the efficacy of a vitrification protocol developed by our group to cryopreserve ovarian tissue. For this, we used ovaries from non-human primates in order to have an animal model close to the clinical setting. Materials and Methods: Ovarian biopsies from five adult baboons were vitrified, warmed and autografted. After five months, follicle survi...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2009
ISSN: 1472-6483
DOI: 10.1016/s1472-6483(10)60136-8